Stir bar-sorptive extraction, solid phase extraction and liquid-liquid extraction for levetiracetam determination in human plasma: comparing recovery rates

  • Priscila Freitas-Lima
  • Flavia Isaura Santi Ferreira
  • Carlo Bertucci
  • Veriano Alexandre Júnior
  • Sônia Aparecida Carvalho Dreossi
  • Leonardo Regis Leira Pereira
  • Américo Ceiki Sakamoto
  • Regina Helena Costa Queiroz
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Publication date
June 2015
Publisher
Universidade de São Paulo
ISSN
1984-8250
Journal
2175-9790

Abstract

<p>Levetiracetam (LEV), an antiepileptic drug (AED) with favorable pharmacokinetic profile, is increasingly being used in clinical practice, although information on its metabolism and disposition are still being generated. Therefore a simple, robust and fast liquid-liquid extraction (LLE) followed by high-performance liquid chromatography method is described that could be used for both pharmacokinetic and therapeutic drug monitoring (TDM) purposes. Moreover, recovery rates of LEV in plasma were compared among LLE, stir bar-sorptive extraction (SBSE), and solid-phase extraction (SPE). Solvent extraction with dichloromethane yielded a plasma residue free from usual interferences such as commonly co-prescribed AEDs, and recoveries around 90% (...

Extracted data

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