Method development and generation of profiles for selected phenolics from apple cultivars used for processed products

Profiles of selected phenolic constituents of 2 apple cultivars, Northern Spy and Ida Red, commonly used for processed products Were compared. Although chlorogenic acid was the principal phenolic in both cultivars, there were significant quantitative differences. A new solid-phase extraction (SPE) and high-performance liquid chromatographic (HPLC) separation using polymeric resins was developed for this experiment. Quantification was by ultra-violet (UV) absorbance using a diode array detector. The performance of this new method was compared with that of a published liquid-liquid extraction (LLE) method, based on linearity of detector response, limits of detection, reproducibility of analysis, and recoveries from spiked apple samples.PT: J; CR: *SAS I INC, 1999, SAS ONL DOC VERS 8 AGBOREGBE T, 1990, J SCI FOOD AGR, V51, P215 AMIOT MJ, 1992, J FOOD SCI, V57, P958 AMIOT MJ, 1993, ACTA HORTIC, V343, P67 ANDRADE PB, 1998, J AGR FOOD CHEM, V46, P968 AWAD MA, 2000, SCI HORTIC-AMSTERDAM, V83, P249 BILYK A, 1988, J LIQ CHROMATOGR, V11, P2829 BURDA S, 1990, J AGR FOOD CHEM, V38, P945 CILLIERS JJL, 1990, J FOOD SCI, V55, P1458 COSETENG MY, 1987, J FOOD SCI, V52, P985 ESCARPA A, 1999, J CHROMATOGR A, V830, P301 JAWORSKI AW, 1987, J AGR FOOD CHEM, V35, P257 LEE CY, 1987, AM J ENOL VITICULT, V38, P277 LEE HS, 1996, HDB FOOD ANAL, V1, P821 LEE HS, 2000, FOOD ANAL HPLC, P775 LISTER CE, 1994, J SCI FOOD AGR, V64, P155 MACHEIX JJ, 1990, FRUIT PHENOLICS, P1 MARQUES L, 1995, ENZYMATIC BROWNING I, P90 MARTINEZ MV, 1995, TRENDS FOOD SCI TECH, V6, P195 MATHEIS G, 1984, J FOOD BIOCHEM, V8, P137 MCRAE KB, 1990, J SCI FOOD AGR, V50, P329 MURATA M, 1995, J JPN SOC FOOD SCI, V42, P820 OSZMIANSKI J, 1990, AM J ENOL VITICULT, V41, P204 PEREZILZARBE J, 1991, Z LEBENSM UNTERS FOR, V192, P551 PODSEDEK A, 2000, EUR FOOD RES TECHNOL, V210, P268 SINGLETON VL, 1986, PHYTOCHEMISTRY, V25, P2127 SPANOS GA, 1990, J AGR FOOD CHEM, V38, P1572 SPANOS GA, 1992, J AGR FOOD CHEM, V40, P1478 TOMASLORENTE F, 1992, J AGR FOOD CHEM, V40, P1800 VAMOSVIGYAZO L, 1981, CRC CRIT REV FOOD SC, V15, P49 WALKER JRL, 1995, ENZYMATIC BROWNING I, P8 WHITAKER JR, 1995, ENZYMATIC BROWNING I, P2 WILSON EL, 1981, J SCI FOOD AGR, V32, P257 ZAWISTOWSKI J, 1991, OXIDATIVE ENZYMES FO, P217; NR: 34; TC: 0; J9: J FOOD SCI; PG: 8; GA: 615PDSource type: Electronic(1