Activity of group-transported horses was evaluated during onboard rest stops to determine if horses derive meaningful rest. A single-deck semi-trailer separated into three compartments was used for all shipments. In Experiment One, twelve video cameras were used to record behavior of horses during five, 16 to 20 h shipments, with a high (397.44kg/m2), medium (348.48 kg/m2) and low (220.91 kg/m2) density group in each shipment. One-hour rest stops occurred after 8 h of transport and prior to unloading, during which two groups were provided water. Movement of each horse visible on video was quantified by counting the number of times the head crossed the vertical and/or horizontal axes of the body at the withers. Mean number of movements per 5...