The subject of the dissertations thesis has been the analysis of utilizing the bulk milk sample as a indicator of the stable environment of cattle of the Holstein. The monitoring was carried out in the Czech Republic in the ŠPZ Žabčice in 55 weeks (between January 2013 and February 2014). Bulk milk samples can be taken as indicators of the quality of the stable environment. We have found out that the average temperature in the stable had a significant influence on the average morning milk yield, on the content of fat, solids non-fat, minerals, active and titration acidities, freezing point of milk (BMM), density, rennet coagulation time, chloride-lactose ratio. The average relative humidity (RH) in the stable conclusively proved the average...