Prokaryotic expression and immunoblotting analysis of truncated NP segments.
- Abulimiti Moming (5775170)
- Daerken Tuoken (5775173)
- Xihong Yue (5775176)
- Wanxiang Xu (5775179)
- Rong Guo (50063)
- Dongliang Liu (653854)
- Yijie Li (653857)
- Zhihong Hu (316172)
- Fei Deng (170132)
- Yujiang Zhang (46399)
- Surong Sun (653860)
View in ORKG Publication date
September 2018
DOI Publisher
Public Library of Science (PLoS)Abstract
(a) SDS-PAGE analysis of expressed pET-32a-NP1, expressed pET-32a-NP3, the pGEX-KG vector (expressing GST-tagged protein as the negative control) and pGEX-KG-NP2. (b) Western blotting of NP1, NP2 and NP3 using rabbit pAbs against r-NP. The arrows represent the three expressed target segments on the gel and the reactive segments in the western blotting analysis.</p