(A) Immuno-FISH performed in human primary fibroblasts (BJ cells) infected for 2 days with the replication-defective HSV-1 virus in1374. HIRA (i), UBN1 (ii), CABIN1 (iii), ASF1a (iv) (green), and HSV-1 genomes (red) were detected. Scale bars = 5 μm. (B) Immuno-FISH performed in adult mouse primary TG neuron cultures infected for 2 days with in1374. Mouse Hira (mHira, green), HSV-1 genomes (red), and nucleus (inset, gray) are detected. Scale bar = 5 μm. (C) Same as (B) but in TG neurons from 6-day HSV-1wt-infected mice. Scale bars = 10 μm. (D) Same as (B) in TG neurons of 28-day HSV-1wt-infected mice. Scale bars = 10 μm. (E) Quantifications of immuno-FISH performed in BJ cells infected with in1374 at early times pi. Data represent the percen...
(A) Schematic localization of the HSV-1 genome and of the loci analyzed by quantitative PCR (qPCR). ...
<p>Vero cells infected with wild type KOS were grown in the presence of 10 μM EdU at 4–8 hpi. Cellul...
Herpes simplex virus type 1 (HSV-1) genomes become associated with structures related to cellular nu...
Immuno-FISH performed in BJ cells not infected (ni) (i, iii, v, vii) or infected for 2 days (ii, iv,...
(A) Immuno-FISH performed in e-H3.1 (i) or e-H3.3 (ii)-expressing BJ cells infected for 2 days with ...
HFt cells were mock infected or infected with 3 PFU/cell of HSV-1EdC or ΔICP0EdC. Cells were fixed a...
<p>(A) Co-localization of HSV-1 genomes with PML protein. TG sections from mock-infected and latentl...
(A) Immuno-FISH performed in TG tissues from pml+/+ and pml-/- infected mice at 28 dpi. Pml, Daxx, A...
Normal or e-H3.3-expressing BJ cells were first transduced with shRNA-expressing lentiviruses before...
(A) WB for the detection of ATRX and HIRA proteins in e-H3.3-expressing BJ cells. Cells were first t...
<p>(A) Immuno-FISH detection of neuronal or viral markers (green) and HSV-1 genomes (red) in RC-cont...
HFt cells were stably transduced to express shRNAs targeting HIRA (shHIRA) or non-targeting control ...
(A) Immuno-FISH performed in e-H3.3-expressing BJ cells transduced with a control (shCTRL) or PML (s...
<p>(<b>A</b>) YFP<sup>+</sup> and YFP<sup>−</sup> cells were identified and captured from DRG sectio...
<p>(A–B) PML signals increase in neuronal and non-neuronal cells during the acute phase. (A) TG sect...
(A) Schematic localization of the HSV-1 genome and of the loci analyzed by quantitative PCR (qPCR). ...
<p>Vero cells infected with wild type KOS were grown in the presence of 10 μM EdU at 4–8 hpi. Cellul...
Herpes simplex virus type 1 (HSV-1) genomes become associated with structures related to cellular nu...
Immuno-FISH performed in BJ cells not infected (ni) (i, iii, v, vii) or infected for 2 days (ii, iv,...
(A) Immuno-FISH performed in e-H3.1 (i) or e-H3.3 (ii)-expressing BJ cells infected for 2 days with ...
HFt cells were mock infected or infected with 3 PFU/cell of HSV-1EdC or ΔICP0EdC. Cells were fixed a...
<p>(A) Co-localization of HSV-1 genomes with PML protein. TG sections from mock-infected and latentl...
(A) Immuno-FISH performed in TG tissues from pml+/+ and pml-/- infected mice at 28 dpi. Pml, Daxx, A...
Normal or e-H3.3-expressing BJ cells were first transduced with shRNA-expressing lentiviruses before...
(A) WB for the detection of ATRX and HIRA proteins in e-H3.3-expressing BJ cells. Cells were first t...
<p>(A) Immuno-FISH detection of neuronal or viral markers (green) and HSV-1 genomes (red) in RC-cont...
HFt cells were stably transduced to express shRNAs targeting HIRA (shHIRA) or non-targeting control ...
(A) Immuno-FISH performed in e-H3.3-expressing BJ cells transduced with a control (shCTRL) or PML (s...
<p>(<b>A</b>) YFP<sup>+</sup> and YFP<sup>−</sup> cells were identified and captured from DRG sectio...
<p>(A–B) PML signals increase in neuronal and non-neuronal cells during the acute phase. (A) TG sect...
(A) Schematic localization of the HSV-1 genome and of the loci analyzed by quantitative PCR (qPCR). ...
<p>Vero cells infected with wild type KOS were grown in the presence of 10 μM EdU at 4–8 hpi. Cellul...
Herpes simplex virus type 1 (HSV-1) genomes become associated with structures related to cellular nu...