Background: Yeast expression systems with altered N-glycosylation are now available to produce glycoproteins with homogenous, defined N-glycans. However, data on the behaviour of these strains in high cell density cultivation are scarce. Results: Here, we report on cultivations under controlled specific growth rate of a GlycoSwitch-Man5 Pichia pastoris strain producing Granulocyte-Macrophage Colony-Stimulating Factor (GM-CSF) at high levels (hundreds of milligrams per liter). We demonstrate that homogenous Man5GlcNAc2 N-glycosylation of the secreted proteins is achieved at all specific growth rates tested. Conclusions: Together, these data illustrate that the GlycoSwitch-Man5 P. pastoris is a robust production strain for homogenously N-glyc...
AbstractPichia pastoris, a methylotrophic yeast, is an established system for the production of hete...
Abstract Background Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) is...
Yeast has advanced as an alternative for mammalian cell culture for the production of recombinant th...
Background: Yeast expression systems with altered N-glycosylation are now available to produce glyco...
Pichia pastoris is used for commercial production of human therapeutic proteins, and genome-scale mo...
Pichia pastoris is a prominent host for recombinant protein production, amongst other things due to ...
Abstract Background The methylotrophic yeast Pichia pastoris is a common host for the production of ...
Yarrowia lipolytica is a dimorphic yeast that efficiently secretes various heterologous proteins and...
The secretory pathway of Pichia pastoris was genetically re-engineered to perform sequential glycosy...
Background: Protein-based therapeutics represent the fastest growing class of compounds in the pharm...
BACKGROUND: Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) is a glyco...
As Pichia pastoris (syn. Komagataella sp.) yeast can secrete pure recombinant proteins at high rates...
The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant glycoprot...
For more than 20 years, the industry has mainly invested in productivity enhancements. Recently, the...
Thesis: Ph. D. in Chemical Engineering Practice, Massachusetts Institute of Technology, Department o...
AbstractPichia pastoris, a methylotrophic yeast, is an established system for the production of hete...
Abstract Background Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) is...
Yeast has advanced as an alternative for mammalian cell culture for the production of recombinant th...
Background: Yeast expression systems with altered N-glycosylation are now available to produce glyco...
Pichia pastoris is used for commercial production of human therapeutic proteins, and genome-scale mo...
Pichia pastoris is a prominent host for recombinant protein production, amongst other things due to ...
Abstract Background The methylotrophic yeast Pichia pastoris is a common host for the production of ...
Yarrowia lipolytica is a dimorphic yeast that efficiently secretes various heterologous proteins and...
The secretory pathway of Pichia pastoris was genetically re-engineered to perform sequential glycosy...
Background: Protein-based therapeutics represent the fastest growing class of compounds in the pharm...
BACKGROUND: Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) is a glyco...
As Pichia pastoris (syn. Komagataella sp.) yeast can secrete pure recombinant proteins at high rates...
The methylotrophic yeast Pichia pastoris is widely used for the production of recombinant glycoprot...
For more than 20 years, the industry has mainly invested in productivity enhancements. Recently, the...
Thesis: Ph. D. in Chemical Engineering Practice, Massachusetts Institute of Technology, Department o...
AbstractPichia pastoris, a methylotrophic yeast, is an established system for the production of hete...
Abstract Background Recombinant human granulocyte-macrophage colony-stimulating factor (rhGM-CSF) is...
Yeast has advanced as an alternative for mammalian cell culture for the production of recombinant th...