Quantitative determination of biologically active compounds in Ginkgo biloba L. leaves during vegetation period using High-Press liquid chromatography method

O. Bukčiūtė master`s thesis/ research supervisor assoc. prof. A. Ževžikovas; Lithuanian University of Health Sciences; Academy of medicine; Faculty of Pharmacy; Departament of Analytical and Toxicological Chemistry – Kaunas. The aim of research. To determine the amount of terpenolactone in Ginkgo biloba L. leaves during vegetation period in different plant growth sites. Aims of work. To determine the amount of terpenolactone in Ginkgo biloba L. leaves gathered from different regions of Lithuania during the vegetation period. To evaluate the changes in the range of terpenolactone during vegetation. To estimate determine the amount of terpenelactone dependences on source of raw material. To evaluate the terpenelactone quantity dependences on climate conditions. Research object. Ginkgo biloba L. leaves, collected in 2014. Research method. The quantity of biologically active compounds (terpenelactone) in Ginkgo biloba L. leaves was determined using High-Press liquid chromatography with refractometric detector. The results and conclusions. In this scientific research was detected the amount of ginkgolides A,B,C and bilobalide in ginkgo leaves collected from 3 different regions of Lithuania during the vegetation period. The average amount of ginkgolide C ranged from 13.954 ± 0.004 to 30.666 ± 0.0002 μg / g, bilobalide – from 429.608 ± 0.007 to 1036.865 ± 0.041 μg / g, ginkgolide B levels ranged from 135.002 ± 0.007 to 210.340 ± 0.011 μg / g, ginkgolide A results ranged from 133.333 ± 0.015 to 367.081 ± 0.082 μg / g. From the results, the total amount of terpenolactone in the entire vegetation period was determined. The lowest amount of terpenolactone was detected in the first month of vegetation – June (1,664 mg /g of terpenolactone), the maximum quantity of terpenolactone was detected in the second month of vegetation (3,975 mg/g of terpenolactone). Comparing the amount of terpenolactone and places, where the ginkgo leaves was collected, was found that the highest concentration of terpenolactone was in leaves from Dzukija. Although the average temperature of 2014 has shown that the highest amount of terpenolactone was detected in the month of the hottest growing season (July), however, no statistically significant results were found between air temperatures and terpenolactone in the evaluation of the data