Cellular GSH content is variable and determines the Cd resistance of individual cells

<p><b>Copyright information:</b></p><p>Taken from "Glutathione and Gts1p drive beneficial variability in the cadmium resistances of individual yeast cells"</p><p></p><p>Molecular Microbiology 2007;66(3):699-712.</p><p>Published online Jan 2007</p><p>PMCID:PMC2167119.</p><p>© 2007 The Authors; Journal compilation © 2007 Blackwell Publishing Ltd</p> Exponential-phase cells were stained with the GSH-specific fluorescent dye mBCl and analysed with fluorescence microscopy or flow cytometry. A. Phase-contrast (left panels) and fluorescence (right panels) images of mBCl-stained wild-type (BY4743) or Δ cells. B. Flow cytometric histograms of mBCl-fluorescence (on a logarithmic scale) in wild-type and Δ cultures; autofluorescence in unstained wild-type cells is also shown. C. mBCl-fluorescence histograms for wild-type cells (B) were gated and sorted into subpopulations containing cells with the lowest ∼20% (○) or highest ∼20% (•) fluorescences, or the entire cell population was gated and ‘sorted’ (▵). Sorted cells were plated onto YPD agar supplemented with Cd(NO). Percentage viability was determined as colony-forming ability after 8 day growth at 30°C, with reference to control growth on Cd-unsupplemented agar. Bars represent means from at least three determinations ± SEM. Typical results from one of four independent experiments are shown