<p>(<b>A</b>) CD4<sup>+</sup> cells were separated by flow cytometric cell sorting into conventional CD4<sup>+</sup>CD25<sup>−</sup> and regulatory CD4<sup>+</sup>CD25<sup>high</sup> T cells as defined by their expression of CD25. (<b>B</b>) Re-analysis of FOXP3 expression in CD4<sup>+</sup>CD25<sup>−</sup> T<sub>conv</sub> (left, grey fill) and CD4<sup>+</sup>CD25<sup>high</sup> T<sub>reg</sub> cells (right, grey fill) post cell sorting. Isotype control (black line). (<b>C</b>) Reduction of proliferation of CD4<sup>+</sup>CD25<sup>−</sup> T<sub>conv</sub> cells stimulated with beads coated with CD3 and CD28 mAbs (black bar) by highly purified CD4<sup>+</sup>CD25<sup>high</sup>FOXP3<sup>+</sup> T<sub>reg</sub> cells from healthy donors (whi...
<p><b>A</b>. CD4<sup>+</sup> T cells were stained with anti-FOXP3, -CD25, -CD45RA and CD127 antibodi...
<p>T cells from direct or transwell-separated iRBC co-cultures were harvested on day 6 of culture an...
<p>CD4+ T cells specific to UL138 (A) or gB (B) from 3 donors were expanded <i>in vitro</i> for 14 d...
<p>(<b>A</b>) Flow cytometric analysis of CD25 and FOXP3 on peripheral blood derived CD4<sup>+</sup>...
<p>(A) CD4<sup>+</sup>CD25<sup>−</sup> cells isolated from a patient with B-cell NHL were co-culture...
<p>(<b>A</b>) Flow cytometric analysis of CD25 and FOXP3 on peripheral blood derived CD4<sup>+</sup>...
<p>CD4<sup>+</sup>CD25<sup>high</sup> T<sub>reg</sub> cells were isolated by flow cytometric cell so...
<p>(<b>A</b>) Strategy of flow cytometric analysis of CCR7 and CD45RA expression on the surface of C...
<p>(<b>a</b>) Representative FoxP3, CTLA-4, CD45RO, CD69 and HLA-DR expression profiles in CD4<sup>+...
<p>(A) A representative FACS analysis of CD25<sup>+</sup> FoxP3<sup>+</sup> cells gated by CD4<sup>+...
<p>(<b>A</b>) Histogram showing CD25 expression pattern of unstimulated CD4<sup>+</sup> T cells (day...
The transcription factor FOXP3 plays a key role in CD4(+)CD25(+) regulatory T cell function and repr...
<p>T cells (n = 5) were stimulated by eight types of DC/tumor (Imm/Mock, OK/Mock, PSK/Mock, OPK/Mock...
Naturally occurring CD4+CD25+ regulatory T cells are thought to play an essential role in the mainte...
<p>Expression of Foxp3<sup>GFP</sup> and CD25 is shown on gated CD4<sup>+</sup> T cells. (A) Analysi...
<p><b>A</b>. CD4<sup>+</sup> T cells were stained with anti-FOXP3, -CD25, -CD45RA and CD127 antibodi...
<p>T cells from direct or transwell-separated iRBC co-cultures were harvested on day 6 of culture an...
<p>CD4+ T cells specific to UL138 (A) or gB (B) from 3 donors were expanded <i>in vitro</i> for 14 d...
<p>(<b>A</b>) Flow cytometric analysis of CD25 and FOXP3 on peripheral blood derived CD4<sup>+</sup>...
<p>(A) CD4<sup>+</sup>CD25<sup>−</sup> cells isolated from a patient with B-cell NHL were co-culture...
<p>(<b>A</b>) Flow cytometric analysis of CD25 and FOXP3 on peripheral blood derived CD4<sup>+</sup>...
<p>CD4<sup>+</sup>CD25<sup>high</sup> T<sub>reg</sub> cells were isolated by flow cytometric cell so...
<p>(<b>A</b>) Strategy of flow cytometric analysis of CCR7 and CD45RA expression on the surface of C...
<p>(<b>a</b>) Representative FoxP3, CTLA-4, CD45RO, CD69 and HLA-DR expression profiles in CD4<sup>+...
<p>(A) A representative FACS analysis of CD25<sup>+</sup> FoxP3<sup>+</sup> cells gated by CD4<sup>+...
<p>(<b>A</b>) Histogram showing CD25 expression pattern of unstimulated CD4<sup>+</sup> T cells (day...
The transcription factor FOXP3 plays a key role in CD4(+)CD25(+) regulatory T cell function and repr...
<p>T cells (n = 5) were stimulated by eight types of DC/tumor (Imm/Mock, OK/Mock, PSK/Mock, OPK/Mock...
Naturally occurring CD4+CD25+ regulatory T cells are thought to play an essential role in the mainte...
<p>Expression of Foxp3<sup>GFP</sup> and CD25 is shown on gated CD4<sup>+</sup> T cells. (A) Analysi...
<p><b>A</b>. CD4<sup>+</sup> T cells were stained with anti-FOXP3, -CD25, -CD45RA and CD127 antibodi...
<p>T cells from direct or transwell-separated iRBC co-cultures were harvested on day 6 of culture an...
<p>CD4+ T cells specific to UL138 (A) or gB (B) from 3 donors were expanded <i>in vitro</i> for 14 d...