Functional analysis of CD4<sup>+</sup>CD25<sup>high</sup>FOXP<sup>+</sup> T<sub>reg</sub> cells.

  • Marc Beyer (133304)
  • Beatrix Schumak (187189)
  • Martin R. Weihrauch (187192)
  • Bettina Andres (187195)
  • Thomas Giese (21715)
  • Elmar Endl (187201)
  • Percy A. Knolle (33375)
  • Sabine Classen (187208)
  • Andreas Limmer (187212)
  • Joachim L. Schultze (133356)
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Publication date
January 2012

Abstract

<p>(<b>A</b>) CD4<sup>+</sup> cells were separated by flow cytometric cell sorting into conventional CD4<sup>+</sup>CD25<sup>−</sup> and regulatory CD4<sup>+</sup>CD25<sup>high</sup> T cells as defined by their expression of CD25. (<b>B</b>) Re-analysis of FOXP3 expression in CD4<sup>+</sup>CD25<sup>−</sup> T<sub>conv</sub> (left, grey fill) and CD4<sup>+</sup>CD25<sup>high</sup> T<sub>reg</sub> cells (right, grey fill) post cell sorting. Isotype control (black line). (<b>C</b>) Reduction of proliferation of CD4<sup>+</sup>CD25<sup>−</sup> T<sub>conv</sub> cells stimulated with beads coated with CD3 and CD28 mAbs (black bar) by highly purified CD4<sup>+</sup>CD25<sup>high</sup>FOXP3<sup>+</sup> T<sub>reg</sub> cells from healthy donors (whi...

Extracted data

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