Postnatal RGM and Neogenin expression in the habenula and fasciculus retroflexus.

<p><i>In situ</i> hybridization (A–C, G–I) and immunohistochemistry (D–F) on coronal mouse brain sections at P5 (A–F) and in the adult (G–I). Sections D–F are counterstained in blue with fluorescent Nissl. (A–C, G–I) At P5 and in the adult, <i>in situ</i> hybridization reveals strong expression of <i>RGMa</i> and <i>RGMb</i> in the medial habenula (MHb). The lateral habenula (LHb) strongly expresses <i>RGMb</i>, while only weak expression of Neogenin is detected in Hb. (D–F) Immunohistochemistry detects strong expression of RGMa and RGMb and weak expression of Neogenin in the fasciculus retroflexus (FR). 3V, third ventricle; CX, cortex; SM, stria medullaris; PVT, paraventricular thalamic nucleus. Scale bars A–I: 200 µm.