Changes in p47 and ROS generation after transfection of Ad-GFP or Ad-XBP1S.

<p>A: Western blot analysis on p47phox levels in the cultured MCs before and after Ad-XBP1S transfection for 48 h (mean ± SEM, n = 5). B: Observation of ROS generation by DHE fluorescent probe assay in the cultured MCs before and after Ad-XBP1S transfection for 48 h (mean ± SEM, n = 8). *<i>p</i><0.05 compared to NG; ^#<i>p</i><0.05 compared with HG; ^##<i>p</i><0.01 compared to HG. C: Western blot analysis on collagen IV and fibronectin levels after transfection of Ad-XBP1S or Ad-XBP1S+xanthine and xanthine oxidase (X⋅XO) for 48 h in the presence of HG. **<i>p</i><0.01 compared to Ad-GFP; ^#<i>p</i><0.05 compared with Ad-XBP1S (mean ± SEM, n = 5).