<i>eya⁺GR</i>, <i>eya^SAGR</i>, and <i>eya^SDEGR</i> rescue eye development and survival in <i>eya</i> mutants.

<p>External (A–E, J–M) and internal (F–I, N–Q) adult eye morphology is fully rescued with genomic transgenes in <i>eya</i> mutants. Homozygous <i>eya²</i> adults carrying a single copy of <i>eya⁺GR</i> (B, G), <i>eya^SAGR</i> (C, H), or <i>eya^SDEGR</i> (D, I) have eye morphology indistinguishable from wild type Canton S (A, F), whereas <i>eya²</i> homozygotes without a rescue construct show complete loss of the compound eye (E). <i>eya^cliIID/Df</i> flies die due to lack of all endogenous <i>eya</i> function, and one copy of <i>eya⁺GR</i> (I, M), <i>eya^SAGR</i> (J, N), or <i>eya^SDEGR</i> (K, O) restores viability and normal eye morphology. Two copies of <i>eya^SDEGR</i> (M, Q) rescue viability and eye morphology in <i>eya</i> null adults equally well as one copy of <i>eya^SDEGR</i>, indicating no gain-of-function phenotype due to the phosphomimetic Eya^SDE protein.