<p><b>A–B</b>. NPCs were treated with 20 ng/mL TNF-α for 4, 8, 24 and 72 h. mRNA expression of IL-6 (A) and LIF (B) were detected by TaqMan Real Time RT-PCR. Data were normalized to GAPDH and presented as fold change compared to control. ** p<0.01 in comparison to control. Results are representative of three independent donors. <b>C–D</b>. NPCs were treated with 20 ng/mL TNF-α for 30 min, 6 h and 24 h. The supernatants were assayed for IL-6 and LIF production by ELISA. Data represent the mean ± SD of triplicate samples from a representative result of three donors. * p<0.05 in comparison to control.</p
<p>-α <b>mRNA expression.</b> Experiment conducted as described in <a href="http://www.plosone.org/a...
<p>ELISA analysis reavealed the diminishing of IL-6 expression on subjecting immDCs, incubated with ...
<p>* <i>vs.</i> unstimulated control (0 ng/mL LPS); § <i>vs.</i> 0.1 ng/mL LPS; † <i>vs.</i> 1 ng/mL...
<p><b>A&B,</b> U251-MG and X1016 cells were treated with TNF-α (10 ng/ml) for the indicated times. R...
<p>Human PBMCs were exposed to IL-6 (10 ng/ml) in combination with sIL-6R (125 ng/ml) (IL-6/sIL-6R) ...
<p><b>A</b>. IL-6 release was determined by ELISA in culture supernatants harvested 3 h, 6 h and 24 ...
<p><b>A</b>. Human NPCs were treated with 20 ng/ml TNF-α for 30 min, 6 h, and 24 h. Expression of ph...
<p>(A) Comparison of basal levels of <i>TNFA</i> mRNA in granulocytes among healthy controls, Group ...
<p>A. TNF-α increased MIF mRNA levels in cultured human NP cells. NP cells were treated with various...
<p>RAW264 cells were incubated in medium containing LPS (1 μg/ml) in the presence or absence of NiCl...
<p>WT, IL-1α and IL-1β KO mice were injected with LPS/GalN at time point 0. Mice were sacrificed at ...
<p>Human synovial fibroblasts were incubated with TNF-α (10 ng/ml) for indicated time intervals. mRN...
<p>Measures of gene expression were performed by quantitative RT-PCR for transcription factors <i>nf...
<p>BMDMs were pulsed with medium alone or PL, LPS, PL+LPS for 30 min, 2 h, 6 h, or 24 h. RNA was iso...
<p>Human PBMCs were pre-exposed to IL-6/sIL-6R for 1 hour. Cells were then stimulated with IL-1β (1 ...
<p>-α <b>mRNA expression.</b> Experiment conducted as described in <a href="http://www.plosone.org/a...
<p>ELISA analysis reavealed the diminishing of IL-6 expression on subjecting immDCs, incubated with ...
<p>* <i>vs.</i> unstimulated control (0 ng/mL LPS); § <i>vs.</i> 0.1 ng/mL LPS; † <i>vs.</i> 1 ng/mL...
<p><b>A&B,</b> U251-MG and X1016 cells were treated with TNF-α (10 ng/ml) for the indicated times. R...
<p>Human PBMCs were exposed to IL-6 (10 ng/ml) in combination with sIL-6R (125 ng/ml) (IL-6/sIL-6R) ...
<p><b>A</b>. IL-6 release was determined by ELISA in culture supernatants harvested 3 h, 6 h and 24 ...
<p><b>A</b>. Human NPCs were treated with 20 ng/ml TNF-α for 30 min, 6 h, and 24 h. Expression of ph...
<p>(A) Comparison of basal levels of <i>TNFA</i> mRNA in granulocytes among healthy controls, Group ...
<p>A. TNF-α increased MIF mRNA levels in cultured human NP cells. NP cells were treated with various...
<p>RAW264 cells were incubated in medium containing LPS (1 μg/ml) in the presence or absence of NiCl...
<p>WT, IL-1α and IL-1β KO mice were injected with LPS/GalN at time point 0. Mice were sacrificed at ...
<p>Human synovial fibroblasts were incubated with TNF-α (10 ng/ml) for indicated time intervals. mRN...
<p>Measures of gene expression were performed by quantitative RT-PCR for transcription factors <i>nf...
<p>BMDMs were pulsed with medium alone or PL, LPS, PL+LPS for 30 min, 2 h, 6 h, or 24 h. RNA was iso...
<p>Human PBMCs were pre-exposed to IL-6/sIL-6R for 1 hour. Cells were then stimulated with IL-1β (1 ...
<p>-α <b>mRNA expression.</b> Experiment conducted as described in <a href="http://www.plosone.org/a...
<p>ELISA analysis reavealed the diminishing of IL-6 expression on subjecting immDCs, incubated with ...
<p>* <i>vs.</i> unstimulated control (0 ng/mL LPS); § <i>vs.</i> 0.1 ng/mL LPS; † <i>vs.</i> 1 ng/mL...