Cysteine residues of Gcf are necessary for the induction of specific antibody response and protection.

  • Sol Kim (331410)
  • Dong-Hyun Joo (331411)
  • Jee-Boong Lee (206440)
  • Byoung-Shik Shim (206456)
  • In Su Cheon (331412)
  • Ji-Eun Jang (206434)
  • Ho-Hyun Song (331413)
  • Kyung-Hyo Kim (206447)
  • Man Ki Song (206460)
  • Jun Chang (206463)
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Publication date
February 2013

Abstract

<p>(A) BALB/c mice were immunized twice with 20 µg of recombinant Gcf or mutant GcfΔCys4 via intranasal route and specific serum antibody titers were measured by ELISA two weeks after immunization. The results represent Log<sub>2</sub> endpoint values from at least five individual mice. (B) Each group of i.n. immunized mice was challenged with 2×10<sup>6</sup> PFU RSV A2 at 4 weeks after boost immunization and the levels of RSV replication in the lungs were determined by plaque assay at day 5 post challenge. <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0032226#s2" target="_blank">Results</a> are expressed as the mean ± SEM (<i>n</i> = 6). The limit of detection was 200 PFU/gram of lungs.</p

Extracted data

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