<p>Schematic representation of p<i>Tv</i>LUC vector constructs (A); <i>Tv</i>PRAC5′UTR, upstream of the <i>Tv</i>PRAC region and containing spliced leader acceptor site; Tubαβ, intergenic region between alpha and beta tubulin genes; NeoR, neomycin phosphotransferase gene; Tubβα, intergenic region between β and α tubulin genes. Representation of the ribosomal promoter region cloned into p<i>Tv-</i>Luc and associated luminescence in Relative Light Units (RLU) detected in the supernatant 48 h after epimastigote transfection with p<i>TvS</i>rDNA-Luc and p<i>TvL</i>rDNA-Luc (B).</p