Effect of CNTF on phosphorylation of signaling proteins in the mHypoE-20/2 neuronal cells.

<p>(A–E) mHypoE-20/2 neurons were serum starved for 12–16 h before treatment with 10 ng/ml (0.45 nM) CNTF (+) or vehicle alone (−) over a 60 min time course. Western blot analysis of cell lysates was performed using phospho-specific antibodies directed against (A) STAT3, (B) JAK2, (C) ERK1/2, (D) Akt, and (E) ACC. All results shown are normalized to G-protein β subunit relative to corresponding control protein levels at each time point and are expressed as mean ± SEM (n = 4 independent experiments, *<i>P</i><0.05). Representative Western blots are shown.