SkQ1 and C<sub>12</sub>TPP stimulate the uncoupling action of FCCP (carbonyl cyanide-p-trifluoromethoxyphenylhydrazone) in rat liver mitochondria.
- Yuri N. Antonenko (241873)
- Ljudmila S. Khailova (406108)
- Dmitry A. Knorre (406109)
- Olga V. Markova (406110)
- Tatyana I. Rokitskaya (241852)
- Tatyana M. Ilyasova (406111)
- Inna I. Severina (406112)
- Elena A. Kotova (241866)
- Yulia E. Karavaeva (406113)
- Anastasia S. Prikhodko (406114)
- Fedor F. Severin (406115)
- Vladimir P. Skulachev (294016)
View in ORKG Publication date
April 2013
DOIAbstract
<p>Mitochondrial potential was assayed by measuring the absorbance of safranin O. 10 nM FCCP was added at 4, 5, 6, 7, 8, and 9 min (curves 2–4). 0.2% ethanol or ethanol solutions leading to 2 µM C<sub>12</sub>TPP (curves 1 and 4) or 2 µM SkQ1 (curve 3) were added at 2 min. Oligomycin (1 µg/ml) was added at 1 min. For other conditions, see <a href="http://www.plosone.org/article/info:doi/10.1371/journal.pone.0061902#s2" target="_blank">Materials and methods</a>.</p