Plasticity of proton leak capacity (state IV⁺) in relation to complex II (CII) in state III respiration.

<p>Isolated liver mitochondria from <i>N. rossii</i> acclimated to 1°C, 0.04 kPa CO₂ (control), <i>n</i> = 9; 7°C, 0.04 kPa CO₂ (warm normocapnic), <i>n</i> = 5; 1°C, 0.2 kPa CO₂ (cold hypercapnic), <i>n</i> = 10; and 7°C 0.2 kPa CO₂ (warm hypercapnic), <i>n</i> = 10, and in mitochondria from control (2°C, 0.04 kPa CO₂, <i>n</i> = 7) and warm-acclimated (9°C, 0.04 kPa CO₂, <i>n</i> = 5) <i>L. squamifrons</i>. White dots represent values at 0°C, grey at 6°C and black at 12°C acute assay temperatures. Values are given as means ± SEM. * indicates a significant difference of state III respiration (horizontal error bars) or of mitochondrial proton leak capacity (vertical error bars) from the 0°C assay within a control/acclimation group (ANOVA, <i>P</i><0.05). The dotted line represents 20% leak of the given state III respiration.