Southern blot analysis of transgenic progeny.

<p>Positive transgenic mice were identified by the presence of the expected 4.7 µg of mouse genomic DNA. <b>A.</b> Positive transgenic mice presenting a strong signal on the Southern blot: Lane 1: line 142. Lane 2: line 131. Lane 3: line 157. <b>B:</b> Positive transgenic mice with a weak signal: Lane 4: line 377. Lane 5: 374, Lane 6: line 396. <b>C:</b> Mice without integrated transgene show no radioactive signal: Lane 7: line 223. Lane 8: Line 275. Lane 9: line 276. <b>D</b>. Negative controls NCrl and line 13 (Lanes 10 and 11) and positive control transgenic mouse line 16 (Lane 12).