Effect of creosol on LPS-induced inflammation in vivo.

<p> Three groups of mice (n = 6 each) were treated with LPS (3 µg/g body weight, given intraperitoneally), LPS (3 µg/g body weight, given intraperitoneally) plus creosol (30 µg/g body weight, given orally 24 h before LPS), or saline alone. At 4 h after LPS injection, serum was collected and assayed for IL-1β (A), IL-6 (B), and TNF-α (C) by ELISA, and, at 24 h, the spleen and liver were collected and assayed, respectively, for expression of COX-2 (D) or NLRP3 (E) by Western blotting. In (A), (B), and (C), the data are expressed as the mean ± SD for three separate experiments, while, in (D) and (E), the results are representative of those obtained in three different experiments and the histogram shows the results for all expressed as the mean ± SD. *indicates a significant difference at the level of <i>p</i><0.05 compared to LPS-injected mice.