Transcriptional silencing of hTERT suppressed the tumorigenecity and invasiveness <i>in vitro</i>.

<p>A: Tumorosphere formation assay. When hTERT was knocked-down, the tumorosphere formation was inhibited <i>in vitro</i>. hTERT knock-down cells were subjected to three dimensional culture condition and were examined for tumor sphere formation after 5 days. B: Quantitative representation of tumorospheres formed in MCF7-HER2 and hTERT knock-down cells. C: Boyden chamber assay. Cell invasiveness was examined by employing Boyden chamber assay. MCF7-HER2 and hTERT knock-down cells were subjected to Boyden chamber assay. Again, less cells invaded the filters when hTERT expression was silenced. D: The cell invasion assay was quantitatively measured in graphic representation.