Immunostaining of β-MHC and osteocalcin along with oil droplets detection after 2 weeks of hMSCs culture in normal growth medium.

<p>Distinct up-regulation of β-MHC, a cardiac marker (green) was observed in the patterned cells (<b>a</b>) but not for the unpatterned cells (<b>d</b>). No signs of osteocalcin expression (<b>b</b>) and oil droplet formation (<b>c</b>) were observed in cells from patterned group. Unpatterned cells expressed osteocalcin abundantly (red) (<b>e</b>), while no oil droplets formation was observed in unpatterned cells (<b>f</b>). The scale bar is 100 µm.