Example of SDS-PAGE of the purified HpASNase double mutant.
- Maristella Maggi (690870)
- Laurent R. Chiarelli (237358)
- Giovanna Valentini (237355)
- Claudia Scotti (200639)
View in ORKG Publication date
February 2015
DOIAbstract
<p>The enzyme was run in parallel with molecular mass standards on a 12% gel and stained with Coomassie Blue R-250. Lane 1: molecular mass markers (97, 66, 45, 31, 21.3 and 14.4 kDa). Lanes 2 and 5: empty lanes. Lane 3 and 4: 250 mM ImOH Nichel fractions. Lane 6: gel filtration, pooled fractions.</p