HMGB1 induced cell migration through activation of MAPK and NF-κB.

<p>Cells pretreated with 2 μM of different inhibitors (A) or transfected with lentiviral based shRNAs targeting ERK (si-ERK), p38 (si-p38), JNK (si-JNK), and p65 (si-p65) (C) were then treated with 100 ng/mL of HMGB1 for 18 h. Cell migration was detected by <i>in vitro</i> scratch assay. (B) (D) Quantification of cell migration was performed by counting the cell numbers in the rectangle in each sample for 4 fields. Data are expressed as mean ± S.E.M. (n ≥ 3) (B) #p<0.05 as compared with negative control (NC) group, in which the cells were treated with PBS. *<i>p</i><0.05,**<i>p</i><0.01, ***<i>p</i><0.001 as compared to HMGB1 100 ng/mL group. (D) #p<0.01 as compared with NC group. *p<0.05 as compared with mock group (lentiviral vector negative control). a*p<0.05 as compared with NC group treated with HMGB1 100 ng/mL. b*p<0.05 as compared with mock group treated with HMGB1 100 ng/mL.