RhoA and Rho kinase inhibitors promote initiation of neurites.

<p>(A) The percentage of cells bearing a neurite was significantly increased at 500 μM of Ibuprofen whereas 10 μM and 100 μM led to no difference to control conditions (3–7 independent experiments, 744 to 2358 neurites measured). (B) Application of cAMP did not change the number of cells bearing a neurite, however neurite lengths were increased to a similar amount compared to Ibuprofen (5 independent experiments, 1151 to 1775 neurites measured). (C) Blocking Rho kinases with increasing levels of Y-27632 resulted in a trend to more cells bearing a neurite. A highly significant effect was observed at 50 μM (3 independent experiments, 732 to 973 neurites measured). (D) Blocking Rho kinases with elevated levels of Fasudil (100 μM) resulted in a highly significant increase in neurite bearing cells (3 independent experiments, 1857 to 3422 neurites measured). **p<0.01 and *p<0.05 against control by Kruskal-Wallis test.