<i>C</i>. <i>pneumoniae</i> interaction with host protein Caprin2.

  • Rhonda Flores (742496)
  • Guangming Zhong (85505)
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Publication date
May 2015

Abstract

<p>(A) Yeasts co-transformed with pEXP-AD502-Caprin2(245–1128) as prey plus pDBLeu-Cpn1027(210–527) as bait (row 1) or the positive control prey pGal4AD-TRAF3 plus bait pGal4DB-LMP1 (row 2) or the negative control prey pGal4AD plus bait pGal4DB-LMP1 (row 3) were grown on plates with selective medium without Leu and Trp (-LW, left column) or without Leu, Trp and His but supplemented with 50mM <i>3-</i>Amino<i>-1</i>,<i>2</i>,<i>4-</i>triazole or 3AT (-LWH 50mM 3AT, middle column) or without Leu, Trp and Uracil (-LWU, right column). Note that bait-prey interactions occurred in rows 1 & 2 but not 3. (B) HEK 293T cells co-transfected with pFLAG-Cpn1027(210–527) and pDsRed vector (RFP alone, left lane), pDsRed-APC4 (RFP-APC4, middle lane) or pDs...

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