RCAN1 effects mitochondrial membrane potential, not volume.
- Heshan Peiris (2795695)
- Michael D. Duffield (2795656)
- Joao Fadista (2795668)
- Claire F. Jessup (2795683)
- Vinder Kashmir (2795692)
- Amanda J. Genders (2795677)
- Sean L. McGee (422110)
- Alyce M. Martin (2795674)
- Madiha Saiedi (2795671)
- Nicholas Morton (2795689)
- Roderick Carter (2795680)
- Michael A. Cousin (160412)
- Alexandros C. Kokotos (2795659)
- Nikolay Oskolkov (779850)
- Petr Volkov (428018)
- Tertius A. Hough (109760)
- Elizabeth M. C. Fisher (245444)
- Victor L. J. Tybulewicz (81533)
- Jorge Busciglio (236409)
- Pinar E. Coskun (2795662)
- Ann Becker (778409)
- Pavel V. Belichenko (778408)
- William C. Mobley (695385)
- Michael T. Ryan (502321)
- Jeng Yie Chan (2795665)
- D. Ross Laybutt (183897)
- P. Toby Coates (2795686)
- Sijun Yang (2795653)
- Charlotte Ling (253302)
- Leif Groop (12005)
- Melanie A. Pritchard (123434)
- Damien J. Keating (339012)
View in ORKG Publication date
May 2016
DOIAbstract
<p>(A) Representative western blots of wild type (WT) and RCAN1<sup>ox</sup> islet protein lysates probed against NDUFA9, SDHA, CORE1, Opa1 and TOM20. Electron microscopy analysis of (B) total mitochondrial area and (C) relative area in WT (n = 8 β-cells) and RCAN1<sup>ox</sup> (n = 6 β-cells). (D) TMRM staining in live cells further demonstrates a lack of difference in mitochondrial volume between WT (n = 43 cells) and RCAN1<sup>ox</sup> (n = 46 cells). (E) Mitochondrial membrane potential measured as mean cell TMRM fluorescence in low and high glucose in WT and RCAN1<sup>ox</sup> cells (n = 17–21 cell/group). Data represents the mean ±SEM, *p<0.05.</p