The effect of LAG-3 silencing on CD4⁺ T-cell killing of <i>Mtb</i> in a differentiated macrophage culture supplemented with CD4⁺ T-cells.

<p>Log₁₀-bacterial burden at 0, 24, 48 72, and 96h post-infection in differentiated <i>Mtb</i>-infected macrophage cultures as well as co-cultures supplemented with CD4⁺ T-cells from blood (A) or lung (B) of <i>Mtb</i>-infected animals. The CD4⁺ T-cells were either untreated, or were silenced for LAG-3 with siRNA. The macrophage only cultures are indicated by solid circles, macrophage cultures supplemented with CD4⁺ T-cells are represented by solid squares, and macrophage cultures supplemented with LAG-3 silenced CD4⁺ T-cells are represented by solid triangles. Statistical significance was determined using a two-way ANOVA in Prism 6, with matched values stacked into sub columns, and using multiple comparisons to compare effects within each row (time) by comparing each experimental condition (macrophage, co-culture or LAG-3 RNAi co-culture) with every other condition at that time-point, with the Tukey multiple comparison test; the mean and SEM are represented by horizontal bars. *p < 0.05, **p < 0.01.