ABSTRACT: We describe a straightforward crystallogenesis protocol leading to the preparation of protein crystals suitable for structure determination that involves protein expression and purification, refolding of the overexpressed protein, search of optimal crystallization conditions and diffraction data collection on native and selenomethionine substituted crystals. The protocol is exemplified with epididymal-specific lipocalin (rLcn6), a newly discovered monomeric protein of 19 kDa that may play an important role in sperm maturation. This protein was cloned from Norway rat (Rattus norVegicus) genome and expressed as insoluble inclusion bodies in Escherichia coli. After refolding of the purified protein, microcrystals were obtained after ...
Inherent difficulties in growing protein crystals are major concerns within structural biology and p...
Efficient cyrstallization of folded proteins from solutions is essential for three dimensional struc...
Protein crystallization in living cells has been observed surprisingly often as a native assembly pr...
ABSTRACT: Protein crystallization is the major bottleneck in the entire process of protein crystallo...
To date, X-ray crystallography remains the gold standard for the determination of macromolecular str...
For every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, a...
While structure determination from micrometre-sized crystals used to represent a challenge, serial X...
During the last decade, the number of three-dimensional structures solved by X-ray crystallography h...
AbstractA systematic approach for improving protein crystals by growing them in the metastable zone ...
In cellulo crystallization is a developing technique to provide crystals for protein structure deter...
This chapter presents in detail the process used in high throughput bacterial production of recombin...
We are developing an alternate strategy for the crystallization of macromolecules that does not, lik...
Third-generation synchrotron sources have revolutionized our understanding of the macromolecular mac...
Structural biology and structural genomics projects routinely rely on recombinantly expressed protei...
We demonstrate the feasibility of growing crystals of protein in volumes as small as 1 nanoliter, Ad...
Inherent difficulties in growing protein crystals are major concerns within structural biology and p...
Efficient cyrstallization of folded proteins from solutions is essential for three dimensional struc...
Protein crystallization in living cells has been observed surprisingly often as a native assembly pr...
ABSTRACT: Protein crystallization is the major bottleneck in the entire process of protein crystallo...
To date, X-ray crystallography remains the gold standard for the determination of macromolecular str...
For every 100 purified proteins that enter crystallization trials, an average of 30 form crystals, a...
While structure determination from micrometre-sized crystals used to represent a challenge, serial X...
During the last decade, the number of three-dimensional structures solved by X-ray crystallography h...
AbstractA systematic approach for improving protein crystals by growing them in the metastable zone ...
In cellulo crystallization is a developing technique to provide crystals for protein structure deter...
This chapter presents in detail the process used in high throughput bacterial production of recombin...
We are developing an alternate strategy for the crystallization of macromolecules that does not, lik...
Third-generation synchrotron sources have revolutionized our understanding of the macromolecular mac...
Structural biology and structural genomics projects routinely rely on recombinantly expressed protei...
We demonstrate the feasibility of growing crystals of protein in volumes as small as 1 nanoliter, Ad...
Inherent difficulties in growing protein crystals are major concerns within structural biology and p...
Efficient cyrstallization of folded proteins from solutions is essential for three dimensional struc...
Protein crystallization in living cells has been observed surprisingly often as a native assembly pr...