ABSTRACT: It is generally believed that water transport across biological membranes is essentially a near-instantaneous process, with water molecules diffusing directly across the membrane as well as through pores such as aquaporins. As a result of these processes by which water can equilibrate across a membrane, a common assumption is that intracellular water is isotopically indistinguishable from extracellular water. To test this assumption directly, we measured the hydrogen isotope ratio of intracellular water in Escherichia coli cells. Our results demonstrate that more than 50 % of the intracellular water hydrogen atoms in log-phase E. coli cells are isotopically distinct from the growth medium water and that these isotopically distinct...
Peculiarities of the vital activity of organisms at different concentrations of the D/H isotope rati...
Dual stable isotope probing has been used to infer rates of microbial biomass production and modes o...
[1] Molecular hydrogen (H2) is one of the most important energy sources for subseafloor chemolithoau...
Hydrogen metabolism was studied in the anaerobic bacterium, Sporomusa sp. strain DMG 58, by measurin...
Metabolic processes result in the release and exchange of H and O atoms from organic material as wel...
Hydrogen atoms from water and food are incorporated into biomass during cellular metabolism and bios...
Microorganisms are involved in all elemental cycles and therefore it is important to study their met...
We report hydrogen isotopic fractionations between water and fatty acids of the sulfate-reducing bac...
Stable isotope probing (SIP) approaches are a suitable tool to identify active organisms in bacteria...
Microorganisms are key players in all elemental cycles, their metabolic activity and potential impac...
Isotopic compositions of carbon-bound hydrogen in individual compounds from eight dierent organisms ...
The hydrogen isotopic composition of fatty acids (FAs) has previously been shown to reflect the meta...
Isotopic compositions of carbon-bound hydrogen in individual compounds from eight different organism...
<p>Rat-1 fibroblasts grown in DMEM were harvested either during exponential growth (30% confluent) o...
Large hydrogen-isotopic (D/H) fractionations between lipids and growth water have been observed in m...
Peculiarities of the vital activity of organisms at different concentrations of the D/H isotope rati...
Dual stable isotope probing has been used to infer rates of microbial biomass production and modes o...
[1] Molecular hydrogen (H2) is one of the most important energy sources for subseafloor chemolithoau...
Hydrogen metabolism was studied in the anaerobic bacterium, Sporomusa sp. strain DMG 58, by measurin...
Metabolic processes result in the release and exchange of H and O atoms from organic material as wel...
Hydrogen atoms from water and food are incorporated into biomass during cellular metabolism and bios...
Microorganisms are involved in all elemental cycles and therefore it is important to study their met...
We report hydrogen isotopic fractionations between water and fatty acids of the sulfate-reducing bac...
Stable isotope probing (SIP) approaches are a suitable tool to identify active organisms in bacteria...
Microorganisms are key players in all elemental cycles, their metabolic activity and potential impac...
Isotopic compositions of carbon-bound hydrogen in individual compounds from eight dierent organisms ...
The hydrogen isotopic composition of fatty acids (FAs) has previously been shown to reflect the meta...
Isotopic compositions of carbon-bound hydrogen in individual compounds from eight different organism...
<p>Rat-1 fibroblasts grown in DMEM were harvested either during exponential growth (30% confluent) o...
Large hydrogen-isotopic (D/H) fractionations between lipids and growth water have been observed in m...
Peculiarities of the vital activity of organisms at different concentrations of the D/H isotope rati...
Dual stable isotope probing has been used to infer rates of microbial biomass production and modes o...
[1] Molecular hydrogen (H2) is one of the most important energy sources for subseafloor chemolithoau...