ROLES OF SURFACE INTERACTION ON CRYPTOSPORIDIUM PARVUM OOCYST TRANSPORT IN SUBSURFACE ENVIRONMENT BY

ii The influence of Cryptosporidium (C.) parvum oocyst and collector surface properties on transport of C. parvum oocysts and microspheres was studied with a radial stagnation point flow (RSPF) cell and 2-dimensional (2-D) micromodels. The quartz or natural organic matter coated quartz surfaces were used as collector in the RSPF cell. The cylindrical silica collectors or silica collectors coated with 0, 10, 20, 50 and, 100 % Fe2O3 patches were firstly fabricated in micromodels. Real time transport of oocyst in RSPF cell and micromodel in electrolyte solutions with systematically varied composition was monitored. Surface polarity and chemical heterogeneity of oocysts surface were characterized by liquid chromatography/nanoelectrospray ionization tandem mass spectrometry (LC-MS/MS), microbial adhesion to hydrocarbon test (MATH), contact angle, and Fourier transform Infrared Spectroscopic (FT-IR) imaging. The most important fundings of this research are the followings: 1) this research developed a method to precisely design complicated yet well-controlled collector properties of porous media and showed the importance of spatial distribution of charged heterogeneou