Phenotypic and genotypic characterization of root nodules rhizobia of Medicago littoralis Rhode and Melilotus indicus (L.) All. growing in the Oasis of Touggourt, Oued Righ Valley, in the Algerian Sahara

Forty rhizobial strains were isolated from root nodules of Medicago littoralis Rhode and Melilotus indicus (L.) harvested from the sandy soils of Touggourt\u2019s oases in the Oued Righ Valley, Algerian Sahara. The isolates were studied for their cultural, biochemical and symbiotic effectiveness. All of them were fast-growing bacteria; utilized a wide range of carbon sources, produced abundant extracellular polysaccharides, tolerated high concentrations of NaCl (up to 2.5\ua0%), grew at temperatures between 28 and 45\ua0\ub0C and at pH values between 4.5 and 9. The isolates were sensitive to the antibiotics kanamycin, tetracycline and rifampicin but showed resistance to neomycin and erythromycin. All the isolates induced the formation of effective nodules on their host plants. On the basis of the physiological, biochemical and symbiotic effectiveness, we selected six strains MD05, MD09, MD12, ML08, ML17 and ML22 for genotypic characterization. Phylogenetic analysis of the selected strains based on 16S ribosomal RNA gene showed that these strains of bacteria were affiliated to the Ensifer meliloti group