Expression profiles of selected celiac disease candidate genes

Introduction: Celiac disease is a multifactorial autoimmune disease that is caused by a response to gluten and related proteins in genetically susceptible individuals. Genetic studies have demonstrated a high association of celiac disease with HLA genes (human leukocyte antigens) II. Class. Approximately 90-95% of the patients have a HLA-DQ2 genotype (DQA1 * 05 / DQB1 * 02), the remaining 5-10% are carriers of HLA-DQ8 (DQA1 * 03 / DQB1 * 03) or inherited only one allele of HLA-DQ2 genotype. Approximately 30% of the healthy population has this genotype and only 1% develops celiac disease. If 30% of healthy individuals have this genotype and do not develop celiac disease, this may be affected by a different level of expression in healthy and diseased individuals. Aims: The aim was to design and test a primer and probe system for QPCR that will amplify all selected HLA-DQA1 and HLA-DQB1 candidate genes at risk for celiac disease and use this system to measure the relative level of expression of risk genes in healthy donors and patients with celiac disease. Methods: The study included 10 patients with recent celiac disease and 15 healthy donors who were selected from the database of the Institute of Medical Genetics of the 3.LF UK based on their genotypes HLA-DQA1 and HLA-DQB1. Patients were first..