Interakce rostlin s pathogeny: význam proteasomu a cytoskeletu

Modification of substrate proteins by ubiquitin is one of the major regulatory events in eukaryotic cells, and plants use this modification to react to exogenous and endogenous cues. Mounting evidence indicates that the ubiquitin-proteasome system is also a key component in response to biotic stressors. This thesis entitled "Plant-pathogen interactions: The role of proteasome and cytoskeleton" reviews the role of proteasome in plant signaling and briefly summarizes interactions between cytoskeleton and pathogen and outlines the role of cytokinin in plant-pathogen interactions. In the experimental part, proteasome-mediated signaling is probed using growth response assay, identification of proteasome substrates and confocal microscopy of cytoskeleton structures. The results present evidence that the employed methodology will be useful for plant-pathogen interaction analyses