Antimikrobiálny efekt semien révy vinnej

The thesis deals with testing the antimicrobial activity of the seed extracts from grapevine, in the research data summary of biologically active substances contained in the vine seed (Vitis vinifera) and brief general characteristic of selected species of microorganisms. In the practical part of thesis was examined antimicrobial effect of seed extracts obtained from grape wine varieties grown in ČR. The first part of thesis deals with the process of producing extract. It was tested nonmethanol extract exposed to oxdiation, fresh methanol and nonmethanol extract in appropriate diluted concentrations. These extracts at 100 %, 50 %, 10 % concentrations were tested by using the agar difusion method, including Escherichia coli, Enterococcus faecalis and Candida tropicalis. All tested microorganisms were inhibited by the grape seed extracts using agar well difusion method and they were cultivated in their optimal conditions for the growth and reproduction after 24 hours and 48 hours. Finally were performed measurings of the inhibition zones