Antimikrobiální působení rostlinných extraktů proti mikroorganismům izolovaným z mléka a mléčných výrobků

The diploma thesis investigates antimicrobial effects of plant extracts (ethereal oil) on microorganisms extracted from milk and milk products. The theoretical part focuses on joghurt and its production, main pathogenic microorganisms and their secondary metabolites affecting health. It presents antimicrobial effects of selected plants and procedures minimising or completely inhibiting the growth of microorganisms. Thyme and anise serve as the evidence for antimicrobial agents; the inhibitory effects of these ethereal plants were observed on the growth of selected microorganism groups (lactic acid bacteria, coliform bacteria, Enterococcus bacteria and yeast) by using disc diffusion test method. The RTS - 1C machine was used to simulate model conditions. Thyme ethereal extract with concentration of 1 and 10 % was used for disc diffusion test method. Anise ethereal extract with concentration of 0,7 % was used for observation of microorganisms' growth in model conditions