Effect of prenylflavonoids on detoxification enzymes in hepatocytes

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Jana Štěpánková Supervisor: Prof. Ing. Barbora Szotáková, Ph.D. Title of Diploma Thesis: The effect of prenylflavonoids on detoxifying enzymes in hepatocytes Prenylated flavonoids are secondary metabolites of many plants, for example hops and fig. They affect the human organism in many positive ways, for example they have antioxidazing, anticancer, anti-inflammatory, estrogenic and antibacterial effects. These effects are even more emphasized due to their prenylated group. Only dietary supplements have sufficient amount of these substances and that is the main reason of patients' purchase. Cytotoxicity of prenylated flavonoids xanthohumol (XH), isoxanthohumol (IXH), 6- prenylnaringenin (6PN) and 8-prenylnaringenin (8PN) towards isolated rat hepatocytes by using the MTT test was studied in this diploma thesis. Furthermore the influence of these prenylflavonoids on the activity and expression of phase I. biotransformation enzymes NAD(P)H:chinonoxidoreductase (NQO1) and aldehydreductase (AKR1A1) was examined. The results of the MTT viability test showed that 6PN and 8PN raise the viability of rat hepatocytes. The viability of hepatocytes was increased by 25 μM XH, however higher..