Methodological study of changes in mechanical properties of collagen tissues from pes equinovarus congenitus

The goal of this thesis was to invent and perform a testing protocol, witch can detect connective tissue mechanical and structural properties simultaneously in patients with clubfoot. Based on literature findings, it was presumed that connective tissue differ between medial and lateral side of foot. Because of low availability of specimen, it was important to draw and mention crucial alterations of testing protokol, for minimization of failed measurments. For reasons above, two specimens diveded into two samples was used for experiment. Described and discused methodics may enable of reader to insigt to drawbacks of this examination. For usability of this protocol, a setting and testing of few hypotheses was performed. One axis tensial testing with SHG microscopy examination was used in combination for experiment. One of the main result was finding, that structural differencies witch were expected, were not distinctive in samples in untensioned state. But, distingtive differencies may be drawed in tensioned samples. This differences for low number of specimen, cannot show any tendence. Results of tensial testing showed, that samples from medial side of foot can have higher toughness and higher fragility. For future acquisition of tendencies in specimen differencies, it is important to set particular..