TRANSPORT OF NSAIDs ACROSS A BLOOD-BRAIN BARRIER IN VITRO MODEL BASED ON CELL LINE PBMEC/C1-2

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Pharmacology and Toxicology Candidate: Mgr. Iveta Nováková Consultant: Prof. PharmDr. František Štaud, Ph.D. Title of Thesis: Transport of NSAIDS across a blood-brain barrier in vitro model based on cell line PBMEC/C1-2 The blood-brain barrier (BBB) has a prominent role in regulation of the transport of substances into and out of the central nervous system (CNS). Partly, the BBB inhibits the entrance of substances harmful for the brain, it regulates the delivery of needed substances and it takes part in efflux of useless substances as well. The equilibrium of these regulation systems is essential for the correct function of the CNS, without which the homeostasis would be disturbed. Non-steroidal anti-inflammatory drugs (NSAIDs) are very well known for their anti- inflammatory effect, for reduction of fever and pain. Due to their bright, everyday usage, some side effects on the brain were observed (sleepiness, giddiness, nausea). This has evoked the question, how NSAIDs can cross the BBB. PBMEC/C1-2 cell monolayer was used as an in vitro model of the BBB. The transport of following NSAIDs was investigated: celecoxib, diclofenac, ibuprofen, lornoxicam, meloxicam, piroxicam and tenoxicam. The experiments were carried out..