Synthesis of steroid phosphoramidates and studies of their properties

Signaling pathway of most important excitatory neurotransmitter - glutamic acid consists of several controllable processes. One of these is modulation of N-methyl-D-aspartate receptor (NMDAR), by endogenous ligands leads to temporary improvement of memory skills and cognitive functions. Positive effects of endogenous or exogenous ligands were also demonstrated on membrane protein Glutamate Carboxypeptidasa II (GCPII). The aim of this work was to prepare steroid derivatives suitable for inhibiting of NMDAR and also GCPII. Steroidal skeleton without substituent at C17 was chosen as a scaffold. The influence of compounds on NMDA receptor will be tested on cultures of human embryonic kidney cells (HEK293) transfected by NR1-1a/NR2B/GFP plasmids of subunits NMDAR. Inhibitory activity on GCPII will be tested on human GCPII with folyl-n-γ-L- glutamic acid as a substrate