Metabolism of anthelmintics in plants

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: František Pavlík Supervisor: Prof. Ing. Barbora Szotáková, Ph.D. Consultant: RNDr. Radka Podlipná, Ph.D. Title of diploma thesis: Metabolism of anthelmintics in plants Anthelmintics enter into environment mainly through excretion of treated animals. They may cause a lot of adverse effects in ecosystem as they can affect non- target animals or they can accumulate in plants. Further, after consumption of these plants by livestock on pastures the resistence of helminths can be induced. Therefore, the present study was designed to find out biotransformation of frequently used anthelmintics flubendazole (FLU), albendazole (ABZ), fenbendazole (FBZ), ivermectine (IVM) and monepantel (MOP) by the common meadow plants harebell (Campanula rotundifolia) and plantain (Plantago lanceolata), which may often come into contact with anthelmintics through the excrements of treated animals. As model systems, suspensions of harebell and plantain cells and in vitro regenerants of plantain were used. There was no toxic effect of anthelmintics for these plants; they were able to metabolize anthelmintics into a wide scale of various compounds. These metabolites were identified by ultrahigh-performance liquid..