In Vitro Study of Interactions of Radiolabeled Monoclonal Antibodies with Epidermal Growth Factor Receptor.

Charles University in Prague, Faculty of Pharmacy in Hradec Kralove Department: Department of Pharmacology and Toxicology Candidate: Zbynek Novy, MSc. Supervisor: Assoc. prof. PharmDr. Frantisek Trejtnar, PhD. Title of doctoral thesis: In vitro study of interactions of radiolabeled monoclonal antibodies with epidermal growth factor receptor Various types of cancer are characterized by overexpression of epidermal growth factor receptor (EGFR) compared to normal tissue. Anti-EGFR antibodies utilize this phenomenon to treat certain oncological diseases. Intensively studied field is verifing of possibility to increase usability of these antibodies by incorporating suitable radionuclides into thiers structures, results can serve in radioimmunodiagnosis or radioimmunotherapy of mentioned malignities. Despite the fact that EGFR recepor and his signaling intracelluar pahtway is object of intense research, there is a lack of knowledge in the area of factors, which can significantly affect binding of radiolabeled antibodies to EGFR in vitro. The aim of this doctoral thesis was to introduce method of radiolabeling anti-EGFR monoclonal antibodies (cetuximab, panitumumab) with iodine-131 and to use this radiolabeled antibodies to determine expression rate of EGFR in four selected cell lines (A431, HaCaT, HCT116..