Green chromatography method development for red dyes determination in soft drinks

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Analytical Chemistry Candidate: Tereza Boháčová Supervisor: Doc. RNDr. Dalibor Šatínský, Ph.D., Dr. Maria Carolina Fernandez Ramos Title of diploma thesis: Development of green chromatography method for red dyes determination in soft drinks Green chromatography HPLC method was used and validated for the simultaneous determination of water-soluble red dyes - Carmoisine E 122, Amaranth E 123, Ponceau 4R E 124, Erythrosine E 127, Red 2G E 128, Allura Red AC E 129. The method was applied for direct determination of Amaranth E 123 and Ponceau 4R E 124 in drinks Syrup black currant (Neli, a.s.), energy drink Kamikaze (Power Life Energy), sparkling wine Bestivo & spritzzante (not shown), Syrup Raspberry light (VESETA spol. s.r.o), and beer Skalák Malina (Pivovar Malý Rohozec). Analytical column Chromolith Performance CN 100 x 4.6 mm was used and the mobile phase contained water / 1% (v/v) acetic acid buffer with addition of amonia for pH adjustment to value 7.0. Successful separation was obtained for all the compounds using an optimized gradient elution within 7 min. Method development was performed at a temperature of 45řC and flow rate 2ml/min. The diode-array detector (DAD) was used to monitor the colorants at the..