Effect of flubendazole on proliferation of colorectal carcinoma cell lines in vitro

Charles University in Prague Faculty of Pharmacy in Hradec Králové Department of Biochemical Sciences Candidate: Kristýna Čáňová Supervisor: Doc. Ing. Barbora Szotáková, Ph.D. Supervisor specialist: RNDr. Věra Králová, Ph.D. Title of rigorous thesis: Effect of flubendazole on proliferation of colorectal carcinoma cell lines in vitro Flubendazole is widely used anthelmintic drug belonging to benzimidazole group. The molecular mechanism of action of flubendazole is based on specific binding to tubulin, which results in disruption of microtubule structure and function and in the interference with the microtubule-mediated transport of secretory vesicles in absorptive tissues of helminths. The microtubule-disrupting properties of benzimidazole derivatives raised recently interest in these compounds as possible anti-cancer agents. Because of available studies and pharmacological statements, it is supposed considerable concentrations of flubendazole in intestinal cells after p.o. administration could be reached, we performed this study to investigate the antiproliferative potential of flubendazole in a panel of intestinal cancer cell lines. The colorectal carcinoma cell lines SW480, SW620 and one cell line NCM460 isolated from normal colon mucosa were treated with different concentrations of flubendazole..