Western blot analysis of iNOS and HO-1 protein expression in aorta of mice with high soluble endoglin levels.

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of Biological and Medical Sciences Western blot analysis of iNOS and HO-1 protein expression in aorta of mice with high soluble endoglin levels Author of diploma thesis: Kristýna Tysoňová Supervisor: PharmDr. Jana Rathouská, Ph.D. Background: The aim of this thesis was to evaluate differences in the expression of iNOS and HO-1 (markers of nitration/oxidative stress) in the aorta of two groups of genetically modified mice on cholesterol diet that differed in levels of human soluble endoglin. Methods: For the study we used 6 months old female transgenic mice on a CBAxC57BL/6J strain backgroud with high level of human soluble endoglin. Using the ELISA analysis, soluble endoglin levels was determined and mice were divided into two groups according to the levels. The study group (Sol-Eng+ ) showed high levels of human soluble endoglin and their sibling mice with low (undetectable) plasma levels of the human endoglin were used as control group. Both groups were fed a special high fat and cholesterol diet for a period of 3 months. Biochemical analysis revealed total cholesterol levels. For detection and quantification of iNOS and HO-1 Western blot analysis was used. Results: After three months on cholesterol diet, the Sol-Eng+..