Evaluation of the activity of potentional antimicrobial substances through the use of microdilution broth method

Charles University in Prague, Faculty of Pharmacy in Hradec Králové Department of: Department of Biological and Medical Sciences Candidate: Mgr. Marie Márová Consultant: Mgr. Marcela Vejsová, Ph.D. Title of Thesis: Evaluation of the activity of potentional antimicrobial substances through the use of microdilution broth method The aim of the present thesis was to test 35 substances and evaluate their antimicrobial activity. The substances were divided by their structure in 10 groups, it concerned carbamates and benzoates of salicylanilides, derivates of mercaptobenzothiazole and derivates of other structures. The substances were tested on 8 bacterial and 8 fungal strains. We used microdilution broth method for testing. This method serves to quantitative determination of sensitivity of the strain to the tested substance, minimal inhibitive concentration (MIC) indicates the sensitivity rate of microb. The holes of microtitration plate were filled with growth medium, tested substance in gradually decreasing concentrations and suspension of the strain. MIC conforms to the concentration of the substance which suppresses the growth of the colony and its hole stayed clear after the incubation. The strongest antibacterial effect was noticed by the substances of the 6th group (thiocarbamates of..