Evaluation of activity of potentional antibiotic substances through the use of microdilution broth method III

Charles Univerzity in Prague Faculty of Pharmacy in Hradec Králové Study program: Pharmacy Author: Tereza Petlánová Supervisor: Mgr. Klára Konečná, Ph.D. Title of diploma thesis: Evaluation of activity of potentional antibiotic substances through the use of microdilution broth method III Background: The aim of this thesis was to test antibiotic substances produced by the Department of Pharmaceutical Chemistry and Drug control Faculty of Pharmacy of Charles University in Hradec Králové. Methods: Substances were tested by using microdilution broth method on eight strains of bacteria: Staphylococcus aureus, Staphylococcus aureus methicilin resistant, Staphylococcus epidermidis, Enterococcus sp., Escherichia coli, Klebsiella pneumoniae, Klebsiella pneumoniae ESBL positive and Pseudomonas aeruginosa. Results: Substances were divided into five groups according to common structural features. The most effective was group of aliphatic derivates of pyrazine-2,3-dicarbonitrile. A groups of cyclic derivates of pyrazine-2,3-dicarbonitrole and cyclic derivates of pyrazine-2- carboxamide were almost ineffective. Conclusion: Of the total of 34 tested compounds was observed antibacterial effect in 13 of them. The most susceptible strains were Enterococcus sp., Straphylococcus aureus and Staphylococcus epidermidis...