Study of complexation equilibria by capillary zone electrophoresis

Capillary zone electrophoresis (CZE) is one of the most widely used analytical methods for separation of chiral analytes. In contrast to the other common chiral separation methods, chiral complexation agent is usually added directly to the background electrolyte to create enantioselective separation environment. Thus, the type and the concentration of chiral selector can be easily varied, which results in high flexibility of separation system. The detail understanding of electrophoretic separation systems with complexation involved is the main goal of this thesis. One of the most important advantages of capillary electrophoresis is existence of its complete mathematical model, which was implemented in several simulation programs. They can provide detail insight into the separation process or predict the separation results. However, none of the available simulators is suitable for complexing separation systems, which limits its applicability for chiral separation systems. For this reason, in the scope of this thesis we introduce the complete mathematical model of electromigration for separation systems with complexation agents. The model was implemented in our dynamic simulator Simul 5 and was verified experimentally. The new version of Simul 5 Complex provides the overall picture about the electrophoretic..