Immunohistochemical detection of macrophages in atherosclerotic lesions brachiocephalic artery I

Sabina Škodová Immunohistochemical detection of macrophages in atherosclerotic lesions brachiocephalic artery. Bachelor thesis Charles University in Prague, Faculty of Pharmacy in Hradec Králové Medical Laboratory Technician Background: The aim of this bachelor thesis was to set imunohistochemic methods for detection of the macrophages in brachiocephalic artery apoE/LDLR receptor deficient mice. This thesis also deals with quantification of expression macrophages in group of mice which were fed with chow diet and in group of mice which were fed with low carbohydrate high protein diet. Methods: For this study were used apoE/LDLR deficient female mice which were separated into two groups (in each group were 6 mice). The first group was fed with chow diet (control group) and the second one was fed with low carbohydrate high protein diet (LCHP). This diet was administered to the mice for 8 weeks. After that the imunohistochemical analysis was performed on the cuts of brachiocephalic artery which uses Immpress method with diaminobenzidin (DAB) for better visibility of macrophages. Afterwards was performed biochemical and stereological analysis. Results: Biochemical analysis showed increased levels of total cholesterol in mice with LCHP diet. Immunohistochemical analysis showed presence of macrophages in..